SAXS/SANS on a model DIS heterocomplex

Aldrex Munsayac Submitted to SASBDB: 2024 May 16
Published in SASBDB:

SASDUS8 – HIV-1 DIS RNA with a CCCCCC apical loop (DIS-C)

HIV-1 dimerization initiation site with a CCCCCC apical loop experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 11 nm3

SASDUT8 – HIV-1 DIS RNA with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension (DIS-Gk)

HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 28 nm3

SASDUU8 – HIV-1 DIS-C:DIS-Gk RNA heterocomplex

HIV-1 dimerization initiation site with a CCCCCC apical loopHIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 20
RgGuinier 3.5 nm
Dmax 13.5 nm
VolumePorod 40 nm3

SASDUV8 – HIV-1 ²H-DIS-C:¹H-DIS-Gk RNA heterocomplex (DIS-Gk contrast matched in 65% v/v D₂O - SANS data)

HIV-1 dimerization initiation site with a CCCCCC apical loopHIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SANS data collected at CG-3, High Flux Isotope Reactor on 2022 Oct 5
RgGuinier 1.6 nm
Dmax 5.3 nm

SASDUW8 – HIV-1 ¹H-DIS-C:²H(42%)-DIS-Gk RNA heterocomplex (DIS-Gk contrast matched in 90% v/v D₂O - SANS data)

HIV-1 dimerization initiation site with a CCCCCC apical loopHIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SANS data collected at CG-3, High Flux Isotope Reactor on 2023 Sep 15
RgGuinier 1.4 nm
Dmax 4.6 nm

SASDUX8 – HIV-1 ²H(42%)-DIS-C:¹H-DIS-Gk RNA heterocomplex (DIS-C contrast matched in 90% v/v D₂O - SANS data)

HIV-1 dimerization initiation site with a CCCCCC apical loopHIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension experimental SAS data
RNAMASONRY model
Sample: HIV-1 dimerization initiation site with a CCCCCC apical loop monomer, 9 kDa RNA
HIV-1 DIS with a GGGGGG apical loop, UCU bulge, and 16 bp helical extension monomer, 21 kDa RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, pH: 7.5
Experiment: SANS data collected at CG-3, High Flux Isotope Reactor on 2023 Sep 15
RgGuinier 2.9 nm
Dmax 9.7 nm