Molecular basis of CRX/DNA recognition and stoichiometry at the Ret4 response element

Srivastava D, Gowribidanur-Chinnaswamy P, Gaur P, Spies M, Swaroop A, Artemyev N
Structure (2024 Jul)

doi: 10.1016/j.str.2024.07.004 		Submitted to SASBDB: 2024 May 23
Published in SASBDB:

SASDU29 – The homeodomain of Cone-rod homeobox protein (CRX) bound to Ret4 of the rhodopsin promoter

Cone-rod homeobox proteinRet4 sense strandRet4 antisense strand experimental SAS data
COOT model
Sample: Cone-rod homeobox protein, 20 kDa Homo sapiens protein
Ret4 sense strand, 6 kDa synthetic construct DNA
Ret4 antisense strand, 6 kDa synthetic construct DNA
Buffer: 20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 25
RgGuinier 2.2 nm
Dmax 6.8 nm
VolumePorod 37 nm3