S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.

Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
J Biol Chem 300(9):107627 (2024 Aug 3)

PMID: 39098536 doi: 10.1016/j.jbc.2024.107627

Submitted to SASBDB: 2024 Jun 17 Published in SASBDB: 2024 Jul 22

SASDVK2 – Core domain of human Cathepsin-G, short truncations to both terminus (Δ22-243Δ253) Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3 RgGuinier 1.9 nm Dmax 6.9 nm VolumePorod 27 nm3

SASDVL2 – Ternary complex consisted of Human Chatepsin G, Neutrophil elastase and S.aureus MAP domain-containing protein Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein MAP domain-containing protein monomer, 13 kDa Staphylococcus aureus (strain … protein Neutrophil elastase monomer, 23 kDa Homo sapiens protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3 RgGuinier 2.8 nm Dmax 9.4 nm VolumePorod 77 nm3

SASDVM2 – Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE) Sample: Neutrophil elastase tetramer, 93 kDa Homo sapiens protein Protein map dimer, 24 kDa Staphylococcus aureus (strain … protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 26 RgGuinier 3.7 nm Dmax 11.0 nm VolumePorod 163 nm3

SASDVF2 – Staphylococcal extracellular adherence protein (Eap) in unbound form Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3 RgGuinier 4.0 nm VolumePorod 64 nm3

SASDVG2 – Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein Cathepsin G tetramer, 101 kDa Homo sapiens protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3 RgGuinier 5.0 nm Dmax 20.2 nm VolumePorod 166 nm3

SASDVH2 – Staphylococcal extracellular adherence protein (Eap) bound to human neutrophil elastase Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein Neutrophil elastase tetramer, 93 kDa Homo sapiens protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Dec 6 RgGuinier 4.1 nm Dmax 10.6 nm VolumePorod 152 nm3

SASDVN2 – S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476) Sample: Protein map monomer, 24 kDa Staphylococcus aureus (strain … protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17 RgGuinier 2.5 nm Dmax 8.5 nm VolumePorod 28 nm3

SASDVP2 – Multimeric complex cosisted of Human Cathepsin G-Neutrophil elastase tetramers bound to S.aureus Protein Map Eap3 and Eap4 domains Sample: Cathepsin G tetramer, 101 kDa Homo sapiens protein Neutrophil elastase tetramer, 93 kDa Homo sapiens protein Eap34 dimer, 48 kDa Staphylococcus aureus (strain … protein Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3 RgGuinier 5.4 nm Dmax 19.4 nm VolumePorod 500 nm3