A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins.

Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
Proc Natl Acad Sci U S A 121(50):e2415220121 (2024 Dec 10)

PMID: 39642200 doi: 10.1073/pnas.2415220121

Submitted to SASBDB: 2024 Jun 5 Published in SASBDB: 2024 Dec 9

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19 RgGuinier 2.6 nm Dmax 11.6 nm VolumePorod 23 nm3

SASDUM9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea Sample: Importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19 RgGuinier 2.6 nm Dmax 13.8 nm VolumePorod 18 nm3

SASDUQ9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1 RgGuinier 2.7 nm Dmax 11.8 nm VolumePorod 22 nm3

SASDUP9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea Sample: Importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1 RgGuinier 3.0 nm Dmax 14.4 nm VolumePorod 31 nm3

SASDUN9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29 RgGuinier 2.7 nm Dmax 11.9 nm VolumePorod 23 nm3

SASDUR9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea Sample: Importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19 RgGuinier 3.0 nm Dmax 12.3 nm VolumePorod 28 nm3

SASDUS9 – Wild-type Elastin-like Polypeptide (ELP) in 0.5 M Urea Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2 RgGuinier 3.1 nm Dmax 10.7 nm VolumePorod 47 nm3

SASDUT9 – Br-labelled Elastin-like Polypeptide (ELP) in 0.5 M Urea Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1 RgGuinier 3.1 nm Dmax 18.4 nm VolumePorod 43 nm3

SASDUV9 – Wild-type Elastin-like Polypeptide (ELP) in 2 M Urea Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2 RgGuinier 3.5 nm Dmax 18.0 nm VolumePorod 53 nm3

SASDUU9 – Br-labelled Elastin-like Polypeptide (ELP) in 2 M Urea Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1 RgGuinier 3.5 nm Dmax 14.8 nm VolumePorod 48 nm3

SASDUW9 – Wild-type Elastin-like Polypeptide (ELP) in 6 M Urea Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2 RgGuinier 3.7 nm Dmax 21.7 nm VolumePorod 52 nm3

SASDUX9 – Br-labelled Elastin-like Polypeptide (ELP) in 6 M Urea Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2 RgGuinier 3.8 nm Dmax 18.1 nm VolumePorod 47 nm3