Potassium cations expand the conformation ensemble of Arabidopsis thaliana β-amylase2 (BAM2).

Sholes A, Asongakap R, Jaconski S, Monroe J, Berndsen CE
MicroPubl Biol 2024 (2024)

PMID: 39109193 doi: 10.17912/micropub.biology.001257

Submitted to SASBDB: 2024 Jun 5 Published in SASBDB: 2024 Jul 12

SASDUZ9 – BAM2 in 100 mM KCl (0.5 mg/mL) Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein Buffer: 50 mM HEPES, 100 mM KCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17 RgGuinier 4.9 nm Dmax 15.0 nm VolumePorod 234 nm3

SASDV22 – BAM2 in 100 mM KCl (1 mg/mL) Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein Buffer: 50 mM HEPES, 100 mM KCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17 RgGuinier 4.7 nm Dmax 15.1 nm VolumePorod 218 nm3

SASDV32 – BAM2 in 100 mM KCl (2 mg/mL) Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein Buffer: 50 mM HEPES, 100 mM KCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17 RgGuinier 4.7 nm Dmax 15.0 nm VolumePorod 220 nm3

SASDUY9 – BAM2 in 100 mM KCl (0.25 mg/mL) Sample: Beta-amylase 2, chloroplastic tetramer, 229 kDa Arabidopsis thaliana protein Buffer: 50 mM HEPES, 100 mM KCl, pH: 7 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 17 RgGuinier 4.9 nm Dmax 16.4 nm VolumePorod 226 nm3