Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.

Prakash A, Moharana K, Wallace SS, Doublié S
Nucleic Acids Res 45(5):2897-2909 (2017 Mar 17)

PMID: 27994037 doi: 10.1093/nar/gkw1282

Submitted to SASBDB: 2016 Nov 4 Published in SASBDB: 2017 Jan 3

SASDBC7 – Human NEI like DNA glycosylase 1 (NEIL1) Sample: Endonuclease 8-like 1 monomer, 45 kDa Homo sapiens protein Buffer: 25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Mar 13 RgGuinier 3.6 nm Dmax 15.0 nm VolumePorod 81 nm3

SASDBD7 – Human Proliferating Cell Nuclear Antigen (PCNA) Sample: Proliferating cell nuclear antigen trimer, 89 kDa Homo sapiens protein Buffer: 25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20 RgGuinier 3.4 nm Dmax 9.7 nm VolumePorod 128 nm3

SASDBB7 – Human NEI like DNA glycosylase 1 (NEIL1) bound to DNA Sample: Endonuclease 8-like 1 monomer, 45 kDa Homo sapiens protein DsDNA monomer, 2 kDa DNA Buffer: 25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20 RgGuinier 3.3 nm Dmax 17.5 nm VolumePorod 92 nm3

SASDBA7 – Human NEI like DNA glycosylase 1 (NEIL1) bound to Proliferating Cell Nuclear Antigen (PCNA) and DNA Sample: Endonuclease 8-like 1 monomer, 45 kDa Homo sapiens protein DsDNA monomer, 2 kDa DNA Proliferating cell nuclear antigen monomer, 30 kDa Homo sapiens protein Buffer: 25mM HEPES 100mM NaCl 1mM DTT, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Jan 20 RgGuinier 3.4 nm Dmax 16.4 nm VolumePorod 113 nm3