The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1.
Crespo I,
Malfois M,
Rienstra J,
Tarrés-Solé A,
van den Berg W,
Weijers D,
Boer DR
Structure
(2025 Mar 7)
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Sample: |
Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL11 - NCD, ALBA on 2019 Dec 3
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RgGuinier |
2.6 |
nm |
Dmax |
6.2 |
nm |
VolumePorod |
59 |
nm3 |
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Sample: |
Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
High Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing dimer, 12 kDa DNA
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL11 - NCD, ALBA on 2019 Dec 3
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RgGuinier |
3.1 |
nm |
Dmax |
8.4 |
nm |
VolumePorod |
67 |
nm3 |
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Sample: |
Auxin response factor 1 dimer, 81 kDa Arabidopsis thaliana protein
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing dimer, 13 kDa DNA
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Buffer: |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 |
Experiment: |
SAXS
data collected at BL11 - NCD, ALBA on 2019 Dec 3
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|
RgGuinier |
3.6 |
nm |
Dmax |
10.5 |
nm |
VolumePorod |
136 |
nm3 |
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