The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1

Isidro Crespo

Submitted to SASBDB: 2024 Aug 1 Published in SASBDB: 2024 Dec 9

SASDVT5 – Marchantia polymorpha Auxin Response Factor 3 Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3 RgGuinier 2.6 nm Dmax 6.2 nm VolumePorod 59 nm3

SASDVU5 – Marchantia polymorpha Auxin Response Factor 3 in complex with high affinity DNA Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein High Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing dimer, 12 kDa DNA Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3 RgGuinier 3.1 nm Dmax 8.4 nm VolumePorod 67 nm3

SASDVV5 – Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA Sample: Auxin response factor 1 dimer, 81 kDa Arabidopsis thaliana protein High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing dimer, 13 kDa DNA Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3 RgGuinier 3.6 nm Dmax 10.5 nm VolumePorod 136 nm3