Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis.

Chandravanshi K, Singh R, Kumar A, Bhange GN, Kumar A, Makde RD
Int J Biol Macromol :136734 (2024 Oct 19)
PMID: 39433196
doi: 10.1016/j.ijbiomac.2024.136734 		Submitted to SASBDB: 2024 Jul 19
Published in SASBDB:

SASDV75 – S9 carboxypeptidase from Bacillus subtilis (4 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
RgGuinier 5.2 nm
Dmax 15.9 nm
VolumePorod 439 nm3

SASDV85 – S9 carboxypeptidase from Bacillus subtilis (6 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
RgGuinier 5.2 nm
Dmax 14.7 nm
VolumePorod 435 nm3

SASDV95 – S9 carboxypeptidase from Bacillus subtilis (8 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
RgGuinier 5.2 nm
Dmax 15.3 nm
VolumePorod 446 nm3