Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation.

Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
IUCrJ (2024 Sep 1)
PMID: 39120045
doi: 10.1107/S2052252524006341 		Submitted to SASBDB: 2024 Aug 1
Published in SASBDB:

SASDVL5 – Small EDRK-rich factor 1 (SERF1a)

Isoform Short of Small EDRK-rich factor 1 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Mar 11
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 9 nm3

SASDVM5 – HTT-3 peptide

HTT3 experimental SAS data
ROSETTA model
Sample: HTT3 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 May 20
RgGuinier 2.0 nm

SASDVN5 – NT17 peptide

NT17 experimental SAS data
ROSETTA model
Sample: NT17 monomer, 2 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Oct 21
RgGuinier 1.2 nm
Dmax 4.0 nm
VolumePorod 5 nm3

SASDVP5 – HTT0 peptide

HTT0 experimental SAS data
ROSETTA model
Sample: HTT0 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2023 Nov 10
RgGuinier 1.5 nm
Dmax 6.0 nm
VolumePorod 4 nm3

SASDVQ5 – HTT1 peptide

HTT1 experimental SAS data
HTT1 Kratky plot
Sample: HTT1 dimer, 8 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Feb 3
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 18 nm3

SASDVR5 – Small EDRK-rich factor 1 (SERF1a) bound to NT17 peptide

Isoform Short of Small EDRK-rich factor 1NT17 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
NT17 dimer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Oct 21
RgGuinier 2.3 nm

SASDVS5 – Small EDRK-rich factor 1 (SERF1a) bound to HTT-3 peptide

Isoform Short of Small EDRK-rich factor 1HTT3 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
HTT3 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 May 20
RgGuinier 2.3 nm
Dmax 7.2 nm
VolumePorod 9 nm3