Integrated structural model of the palladin-actin complex using XL-MS, docking, and SAXS

Rachel Sargent Submitted to SASBDB: 2024 Aug 22
Published in SASBDB:

SASDVD6 – The Ig-like C2-type 4 domain (Ig4WT) of Palladin

Palladin experimental SAS data
SASREF model
Sample: Palladin monomer, 12 kDa Mus musculus protein
Buffer: 20 mM HEPES pH 7.4, 1 mM DTT, 100 mM NaCl, pH:
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2023 Aug 29
RgGuinier 1.7 nm
Dmax 6.8 nm
VolumePorod 18 nm3

SASDVE6 – The Ig-like C2-type 3 domain (Ig3WT) of Palladin

Palladin experimental SAS data
SASREF model
Sample: Palladin monomer, 12 kDa Mus musculus protein
Buffer: 20 mM HEPES pH 7.4, 1 mM DTT, 100 mM NaCl, pH:
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2023 Aug 29
RgGuinier 1.6 nm
Dmax 6.7 nm
VolumePorod 18 nm3

SASDVF6 – The Ig-like C2-type 3 and Ig-like C2-type 4 domains (Ig34WT) of Palladin

Palladin experimental SAS data
The Ig-like C2-type 3 and Ig-like C2-type 4 domains (Ig34WT) of Palladin Rg histogram
Sample: Palladin monomer, 27 kDa Mus musculus protein
Buffer: 20 mM HEPES pH 7.4, 1 mM DTT, 100 mM NaCl, pH:
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2023 Aug 29
RgGuinier 2.8 nm
Dmax 12.3 nm
VolumePorod 29 nm3