NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism.

Brosey CA, Shen R, Tainer JA
EMBO J (2025 Jan 13)

PMID: 39806100 doi: 10.1038/s44318-024-00360-6

Submitted to SASBDB: 2024 Sep 7 Published in SASBDB: 2025 Jan 15

SASDV47 – Peptide-linked fusion protein of Apoptosis-inducing factor 1 AIF(104-613) point mutant W196A and the N-terminal segment of Mitochondrial intermembrane space import and assembly protein 40 CHCHD4(1-45) Sample: Mitochondrial intermembrane space import and assembly protein 40 dimer, 12 kDa Homo sapiens protein Apoptosis-inducing factor 1, mitochondrial dimer, 113 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 28 RgGuinier 3.9 nm Dmax 12.9 nm VolumePorod 289 nm3

SASDVY6 – Wild-type full-length Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26 RgGuinier 2.9 nm Dmax 9.2 nm VolumePorod 56 nm3

SASDVZ6 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) triple mutant I12A/F14A/H20A (AIA-A) Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26 RgGuinier 3.1 nm Dmax 10.5 nm VolumePorod 62 nm3

SASDV27 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) point mutant H20D Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26 RgGuinier 3.2 nm Dmax 11.2 nm VolumePorod 66 nm3

SASDV37 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) point mutant L28D Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26 RgGuinier 2.8 nm Dmax 9.2 nm VolumePorod 52 nm3