Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders

Kroupova A, Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
Nature Communications 15(1) (2024 Oct 15)

doi: 10.1038/s41467-024-52871-9 		Submitted to SASBDB: 2024 Aug 29
Published in SASBDB:

SASDVN6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-VcN

Cereblon-midi experimental SAS data
Cereblon-midi Kratky plot
Sample: Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer: 20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 29
RgGuinier 2.3 nm
Dmax 9.1 nm
VolumePorod 63 nm3

SASDVP6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-AcQ

Cereblon-midi experimental SAS data
Cereblon-midi Kratky plot
Sample: Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer: 20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 29
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 62 nm3