SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in Aspergillus fumigatus siderophore biosynthesis

Poonsiri T, Stransky J, Demitri N, Haas H, Cianci M, Benini S
Journal of Structural Biology: X 11:100119 (2025 Jun)

doi: 10.1016/j.yjsbx.2024.100119

Submitted to SASBDB: 2024 Oct 21 Published in SASBDB: 2025 Jan 2

SASDV98 – Full-length N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF Sample: N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF tetramer, 223 kDa Aspergillus fumigatus (strain … protein Buffer: 50 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jul 7 RgGuinier 4.0 nm Dmax 19.0 nm VolumePorod 412 nm3

SASDVB8 – C-terminal truncation mutant of N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF Sample: N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462) dimer, 107 kDa Aspergillus fumigatus (strain … protein Buffer: 50 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2023 Jan 18 RgGuinier 3.5 nm Dmax 15.0 nm VolumePorod 189 nm3

SASDVA8 – The N-terminal domain of N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF Sample: N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF monomer, 25 kDa Aspergillus fumigatus (strain … protein Buffer: 50 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2023 Jan 18 RgGuinier 2.2 nm Dmax 8.9 nm VolumePorod 30 nm3