Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.

Cantero-Camacho Á, Fan L, Wang YX, Gallego J
RNA 23(9):1465-1476 (2017 Sep)
PMID: 28630140
doi: 10.1261/rna.060632.117 		Submitted to SASBDB: 2016 Nov 23
Published in SASBDB:

SASDBM7 – Subdomain SL1 of hepatitis C virus RNA domain 3'X at low ionic strength

Subdomain SL1 of hepatitis C virus experimental SAS data
DAMMIN model
Sample: Subdomain SL1 of hepatitis C virus monomer, 15 kDa Hepatitis C virus RNA
Buffer: 10mM Tris 0.1 mM EDTA, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11
RgGuinier 2.3 nm
Dmax 8.1 nm

SASDBN7 – Subdomain SL2' of hepatitis C virus RNA domain 3'X at low ionic strength

Subdomain SL2' of hepatitis C virus domain 3'X experimental SAS data
DAMMIN model
Sample: Subdomain SL2' of hepatitis C virus domain 3'X monomer, 18 kDa Hepatitis C virus RNA
Buffer: 10mM Tris 0.1 mM EDTA, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11
RgGuinier 2.8 nm
Dmax 10.0 nm

SASDBP7 – Domain 3'X of hepatitis C virus RNA at low ionic strength

Domain 3'X of hepatitis C virus experimental SAS data
DAMMIN model
Sample: Domain 3'X of hepatitis C virus monomer, 32 kDa Hepatitis C virus RNA
Buffer: 10mM Tris 0.1 mM EDTA, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11
RgGuinier 3.7 nm
Dmax 14.8 nm

SASDBQ7 – Domain 3'X of hepatitis C virus RNA at higher ionic strength

Domain 3'X of hepatitis C virus experimental SAS data
DAMMIN model
Sample: Domain 3'X of hepatitis C virus dimer, 63 kDa Hepatitis C virus RNA
Buffer: 10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Center for Cancer Research, National Cancer Institute on 2015 Nov 20
RgGuinier 6.0 nm
Dmax 24.4 nm

SASDBR7 – Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength

Subdomain SL2' of hepatitis C virus domain 3'X experimental SAS data
DAMMIN model
Sample: Subdomain SL2' of hepatitis C virus domain 3'X dimer, 36 kDa Hepatitis C virus RNA
Buffer: 10mM Tris 0,1mM EDTA 2mM MgCl2, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11
RgGuinier 4.1 nm
Dmax 16.9 nm