Early events in G-quadruplex folding captured by time-resolved small-angle X-ray scattering.

Monsen RC, Sabo TM, Gray R, Hopkins JB, Chaires JB
Nucleic Acids Res 53(3) (2025 Jan 24)

PMID: 39883009 doi: 10.1093/nar/gkaf043

Submitted to SASBDB: 2024 Nov 28 Published in SASBDB: 2025 Feb 10

SASDW43 – Telomere DNA G-quadruplex (2GKU) at neutral pH Sample: Telomere DNA G-quadruplex Hybrid-1 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11 RgGuinier 1.2 nm Dmax 3.8 nm VolumePorod 8 nm3

SASDW53 – Telomere DNA G-quadruplex (2JSL) at neutral pH Sample: Telomere DNA G-quadruplex Hybrid-2 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11 RgGuinier 1.2 nm Dmax 3.8 nm VolumePorod 8 nm3

SASDW63 – Telomere DNA G-quadruplex (2GKU) at neutral pH with LiCl Sample: Telomere DNA G-quadruplex Hybrid-1 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Li2HPO4, 2 mM LiH2PO4, 1 mM EDTA, 185 mM LiCl, pH 7.2, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11 RgGuinier 1.2 nm Dmax 4.3 nm VolumePorod 7 nm3

SASDW73 – Telomere DNA G-quadruplex (2JSL) at neutral pH with LiCl Sample: Telomere DNA G-quadruplex Hybrid-2 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Li2HPO4, 2 mM LiH2PO4, 1 mM EDTA, 185 mM LiCl, pH 7.2, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12 RgGuinier 1.3 nm Dmax 5.1 nm VolumePorod 8 nm3

SASDW83 – Telomere DNA G-quadruplex (2GKU) at alkaline pH Sample: Telomere DNA G-quadruplex Hybrid-1 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 11.5, pH: 11.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12 RgGuinier 2.4 nm Dmax 8.3 nm VolumePorod 12 nm3

SASDW93 – Telomere DNA G-quadruplex (2JSL) at alkaline pH Sample: Telomere DNA G-quadruplex Hybrid-2 form monomer, 8 kDa synthetic construct DNA Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 11.5, pH: 11.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12 RgGuinier 2.4 nm Dmax 8.7 nm VolumePorod 12 nm3