Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux

Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
Journal of Applied Crystallography 58(2) (2025 Feb 11)

doi: 10.1107/S1600576725000779 		Submitted to SASBDB: 2024 Dec 9
Published in SASBDB:

SASDWA3 – Ovalbumin, Monomer from SEC-SANS

Ovalbumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: 100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22
RgGuinier 2.0 nm
Dmax 6.5 nm

SASDWB3 – Bovine serum albumin, monomer from SEC-SANS

Albumin (natural variant A214T) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Albumin (natural variant A214T) monomer, 66 kDa Bos taurus protein
Buffer: 100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22
RgGuinier 2.6 nm
Dmax 8.7 nm

SASDWC3 – Apoferritin, from SEC-SANS

Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer: 100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22
RgGuinier 5.4 nm
Dmax 23.1 nm

SASDWD3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (hydrogenated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
RgGuinier 4.9 nm
Dmax 14.4 nm

SASDWE3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
RgGuinier 4.3 nm
Dmax 11.7 nm

SASDWF3 – Circadian clock protein KaiC (hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
RgGuinier 4.4 nm
Dmax 11.9 nm