IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state

Imamura H, Honda S
FEBS Letters (2025 Mar 17)

doi: 10.1002/1873-3468.70031

Submitted to SASBDB: 2024 Dec 19 Published in SASBDB: 2025 Mar 17

SASDW44 – Immunoglobulin G4 (pH 2, 0.2 M NaCl), SEC-SAXS data Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19 RgGuinier 4.1 nm Dmax 14.0 nm VolumePorod 238 nm3

SASDW54 – Immunoglobulin G1 (pH 2, 0.2 M NaCl), SEC-SAXS data Sample: Humanized immunoglobulin G1 monoclonal antibody (trastuzumab) monomer, 148 kDa Mouse/human protein Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 May 20 RgGuinier 4.2 nm Dmax 12.9 nm VolumePorod 240 nm3

SASDW64 – Immunoglobulin G4 (pH 7) Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein Buffer: 10 mM sodium phosphate, pH: 7 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19 RgGuinier 4.8 nm Dmax 15.6 nm VolumePorod 211 nm3