Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N

Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J
Journal of Biological Chemistry :108223 (2025 Jan)

doi: 10.1016/j.jbc.2025.108223

Submitted to SASBDB: 2025 Jan 9 Published in SASBDB: 2025 Jan 30

SASDWK4 – Shewanella benthica hemoglobin Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 May 1 RgGuinier 1.8 nm Dmax 5.8 nm VolumePorod 30 nm3

SASDWL4 – Shewanella benthica hemoglobin 0.101 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 70 nm3

SASDWM4 – Shewanella benthica hemoglobin 0.101 MPa (final measurement after end of pressure series) Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 70 nm3

SASDWN4 – Shewanella benthica hemoglobin 50 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 70 nm3

SASDWP4 – Shewanella benthica hemoglobin 100 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 6.4 nm VolumePorod 50 nm3

SASDWQ4 – Shewanella benthica hemoglobin 150 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.1 nm Dmax 6.5 nm VolumePorod 50 nm3

SASDWR4 – Shewanella benthica hemoglobin 200 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.1 nm Dmax 6.5 nm VolumePorod 41 nm3

SASDWS4 – Shewanella benthica hemoglobin 250 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.0 nm Dmax 8.0 nm VolumePorod 28 nm3

SASDWT4 – Shewanella benthica hemoglobin 350 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 9.0 nm VolumePorod 30 nm3

SASDWU4 – Shewanella benthica hemoglobin K111I 0.101 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.9 nm Dmax 7.0 nm VolumePorod 38 nm3

SASDWV4 – Shewanella benthica hemoglobin K111I 0.101 MPa (final measurement after end of pressure series) Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.7 nm Dmax 6.3 nm VolumePorod 28 nm3

SASDWW4 – Shewanella benthica hemoglobin K111I 50 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.6 nm Dmax 6.0 nm VolumePorod 24 nm3

SASDWX4 – Shewanella benthica hemoglobin K111I 100 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.6 nm Dmax 5.7 nm VolumePorod 25 nm3

SASDWY4 – Shewanella benthica hemoglobin K111I 150 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.7 nm Dmax 5.9 nm VolumePorod 24 nm3

SASDWZ4 – Shewanella benthica hemoglobin K111I 200 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.7 nm Dmax 6.8 nm VolumePorod 24 nm3

SASDW25 – Shewanella benthica hemoglobin K111I 250 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.8 nm Dmax 7.0 nm VolumePorod 29 nm3

SASDW35 – Shewanella benthica hemoglobin K111I 300 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.8 nm Dmax 9.0 nm VolumePorod 28 nm3

SASDW45 – Shewanella benthica hemoglobin K111I 350 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, K111I), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.9 nm Dmax 10.0 nm VolumePorod 29 nm3

SASDW55 – Shewanella benthica hemoglobin Y34F 0.101 MPa Sample: Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.3 nm Dmax 6.9 nm VolumePorod 75 nm3

SASDW65 – Shewanella benthica hemoglobin Y34F 100 MPa Sample: Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.3 nm Dmax 7.0 nm VolumePorod 66 nm3

SASDW75 – Shewanella benthica hemoglobin Y34F 150 MPa Sample: Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 61 nm3

SASDW85 – Shewanella benthica hemoglobin Y34F 200 MPa Sample: Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.0 nm Dmax 8.5 nm VolumePorod 24 nm3

SASDW95 – Shewanella benthica hemoglobin Y34F 250 MPa Sample: Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.1 nm Dmax 6.6 nm VolumePorod 49 nm3

SASDWA5 – Shewanella benthica hemoglobin Y108A 0.101 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.4 nm Dmax 4.0 nm VolumePorod 20 nm3

SASDWB5 – Shewanella benthica hemoglobin Y108A 0.101 MPa (replicate) Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.4 nm Dmax 4.0 nm VolumePorod 20 nm3

SASDWC5 – Shewanella benthica hemoglobin Y108A 50 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.5 nm Dmax 4.5 nm VolumePorod 20 nm3

SASDWD5 – Shewanella benthica hemoglobin Y108A 100 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.5 nm Dmax 4.8 nm VolumePorod 20 nm3

SASDWE5 – Shewanella benthica hemoglobin Y108A 150 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.6 nm Dmax 6.0 nm VolumePorod 21 nm3

SASDWF5 – Shewanella benthica hemoglobin Y108A 200 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 1.8 nm Dmax 8.8 nm VolumePorod 24 nm3

SASDWG5 – Shewanella benthica hemoglobin Y108A 250 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.1 nm Dmax 9.5 nm VolumePorod 25 nm3

SASDWH5 – Shewanella benthica hemoglobin Y108A 300 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.2 nm Dmax 12.0 nm VolumePorod 26 nm3

SASDWJ5 – Shewanella benthica hemoglobin Y108A 350 MPa Sample: Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein Buffer: 14 mM Tris, 6 mM potassium phosphate, pH: 7 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18 RgGuinier 2.3 nm Dmax 11.0 nm VolumePorod 31 nm3