Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.

Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
Elife 6 (2017 Mar 23)

PMID: 28332978 doi: 10.7554/eLife.22510

Submitted to SASBDB: 2016 Nov 26 Published in SASBDB: 2017 May 2

SASDBU7 – DNA binding domain (DBD) of chromo domain-containing protein 1 (Chd1: 1009-1274) Sample: Chromodomain helicase DNA binding domain monomer, 31 kDa Saccharomyces cerevisiae protein Buffer: 50mM Hepes 150mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 20 RgGuinier 2.6 nm Dmax 8.3 nm

SASDBV7 – Chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 133-1305) Sample: Chromodomain helicase DNA binding domain monomer, 135 kDa Saccharomyces cerevisiae protein Buffer: 50mM Hepes 150mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2008 Nov 30 RgGuinier 4.2 nm Dmax 15.4 nm VolumePorod 280 nm3

SASDBW7 – N-terminal and chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 1-1305) Sample: Chromodomain helicase DNA binding domain monomer, 150 kDa Saccharomyces cerevisiae protein Buffer: 50mM Hepes 150mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30 RgGuinier 4.9 nm Dmax 16.0 nm VolumePorod 340 nm3

SASDBX7 – Chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 133-1010) Sample: Chromodomain helicase DNA binding domain monomer, 102 kDa Saccharomyces cerevisiae protein Buffer: 50mM Hepes 150mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30 RgGuinier 4.1 nm Dmax 16.1 nm VolumePorod 190 nm3

SASDBY7 – N-terminal and chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 1-1010) Sample: Chromodomain helicase DNA binding domain monomer, 117 kDa Saccharomyces cerevisiae protein Buffer: 50mM Hepes 150mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 30 RgGuinier 4.5 nm Dmax 12.0 nm VolumePorod 228 nm3