Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS

Verdonk C, Agostino M, Eto K, Hall D, Bond C, Ramsay J
Nucleic Acids Research 53(6) (2025 Apr 02)

doi: 10.1093/nar/gkaf249 		Submitted to SASBDB: 2025 Feb 11
Published in SASBDB:

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data
OTHER model
Sample: Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein
AttP_8 40-mer DNA dimer, 25 kDa DNA
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3