YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property

Krishan Gopal

Submitted to SASBDB: 2017 Jan 5 Published in SASBDB: 2021 Sep 20

SASDBL9 – Putative DNA binding protein YeeF-CT from Bacillus subtilis (amino acids 528-669) Sample: Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17 RgGuinier 2.7 nm Dmax 9.5 nm VolumePorod 55 nm3

SASDBM9 – YezG: a cognate immunity protein of YeeF from Bacillus subtilis Sample: YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17 RgGuinier 2.1 nm Dmax 6.1 nm VolumePorod 46 nm3

SASDBN9 – Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis Sample: Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 18 RgGuinier 4.0 nm Dmax 14.1 nm VolumePorod 115 nm3