Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex.
Upla P,
Kim SJ,
Sampathkumar P,
Dutta K,
Cahill SM,
Chemmama IE,
Williams R,
Bonanno JB,
Rice WJ,
Stokes DL,
Cowburn D,
Almo SC,
Sali A,
Rout MP,
Fernandez-Martinez J
Structure
25(3):434-445
(2017 Mar 7)
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Sample: |
Nucleoporin POM152 monomer, 12 kDa Saccharomyces cerevisiae protein
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Buffer: |
10mM HEPES, 150mM NaCl, 10%(v/v) glycerol, 5mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 Apr 12
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RgGuinier |
1.8 |
nm |
Dmax |
6.7 |
nm |
VolumePorod |
18 |
nm3 |
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Sample: |
Nucleoporin POM152 monomer, 24 kDa Saccharomyces cerevisiae protein
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Buffer: |
10mM HEPES, 150mM NaCl, 10%(v/v) glycerol, 5mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 Apr 12
|
|
RgGuinier |
2.7 |
nm |
Dmax |
9.4 |
nm |
VolumePorod |
23 |
nm3 |
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Sample: |
Nucleoporin POM152 monomer, 12 kDa Saccharomyces cerevisiae protein
|
Buffer: |
10mM HEPES, 150mM NaCl, 10%(v/v) glycerol, 5mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 Apr 12
|
|
RgGuinier |
2.8 |
nm |
Dmax |
7.9 |
nm |
VolumePorod |
57 |
nm3 |
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Sample: |
Nucleoporin POM152 monomer, 26 kDa Saccharomyces cerevisiae protein
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Buffer: |
10mM HEPES, 150mM NaCl, 10%(v/v) glycerol, 5mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 Apr 12
|
|
RgGuinier |
3.0 |
nm |
Dmax |
10.5 |
nm |
VolumePorod |
28 |
nm3 |
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Sample: |
Nucleoporin POM152 monomer, 49 kDa Saccharomyces cerevisiae protein
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Buffer: |
10mM HEPES, 150mM NaCl, 10%(v/v) glycerol, 5mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 Apr 12
|
|
RgGuinier |
4.3 |
nm |
Dmax |
15.4 |
nm |
VolumePorod |
67 |
nm3 |
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