Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20.

Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
Sci Rep 7(1):5798 (2017 Jul 19)
PMID: 28724964
doi: 10.1038/s41598-017-06133-y
Submitted to SASBDB: 2017 Jan 12
Published in SASBDB:

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 51 nm3

SASDBE9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 49 nm3

SASDBF9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 46 nm3

SASDBG9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
RgGuinier 2.3 nm
Dmax 8.9 nm
VolumePorod 44 nm3

SASDBH9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of molybdate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
RgGuinier 2.3 nm
Dmax 9.2 nm
VolumePorod 46 nm3