Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.

White MA, Tsalkova T, Mei FC, Cheng X
Cells 9(1) (2019 Dec 21)

PMID: 31877746 doi: 10.3390/cells9010035

Submitted to SASBDB: 2017 Jul 27 Published in SASBDB: 2019 Dec 2

SASDCQ6 – Rap guanine nucleotide exchange factor 3 (isoform3) - apo form Sample: Rap guanine nucleotide exchange factor 3 monomer, 100 kDa Homo sapiens protein Buffer: 1mM EDTA, 10mM DTT, 500mM NaCl, and 10mM Tris, pH: 9 Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2012 Sep 7 RgGuinier 3.4 nm Dmax 11.0 nm VolumePorod 180 nm3

SASDCR6 – Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP Sample: Rap guanine nucleotide exchange factor 3 (dimer) dimer, 200 kDa Homo sapiens protein Buffer: 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, and 10mM Tris, pH: 9 Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2012 Jan 30 RgGuinier 5.3 nm Dmax 15.7 nm VolumePorod 415 nm3

SASDCS6 – Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP Sample: Rap guanine nucleotide exchange factor 3 monomer, 100 kDa Homo sapiens protein RAS related protein 1b monomer, 18 kDa Mus musculus protein Buffer: 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, and 10mM Tris, pH: 9 Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2013 Apr 1 RgGuinier 4.1 nm Dmax 14.2 nm VolumePorod 207 nm3