A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover.

Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
Nat Commun 8(1):1008 (2017 Oct 18)

PMID: 29044096 doi: 10.1038/s41467-017-01103-4

Submitted to SASBDB: 2017 Mar 21 Published in SASBDB: 2017 Oct 20

SASDC82 – Rab family protein CtRoco nucleotide free Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16 RgGuinier 5.0 nm Dmax 18.4 nm VolumePorod 440 nm3

SASDC92 – Rab family protein CtRoco GppNHp Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16 RgGuinier 6.2 nm Dmax 25.3 nm VolumePorod 354 nm3

SASDCA2 – Rab family protein CtRoco GDP Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17 RgGuinier 5.9 nm Dmax 22.4 nm VolumePorod 491 nm3

SASDCB2 – Rab family protein CtRoc-COR nucleotide free Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1 RgGuinier 3.4 nm Dmax 10.0 nm VolumePorod 211 nm3

SASDCC2 – Rab family protein CtRoc-COR GDP Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µ M GDP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1 RgGuinier 3.5 nm Dmax 10.0 nm VolumePorod 197 nm3

SASDCD2 – Rab family protein CtRoc-COR GppNHp Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1 RgGuinier 3.4 nm Dmax 10.5 nm VolumePorod 154 nm3

SASDCE2 – Rab family protein CtRoco L487A GDP Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17 RgGuinier 5.9 nm Dmax 21.8 nm VolumePorod 469 nm3

SASDCF2 – Rab family protein CtRoco L487A GppNHp Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16 RgGuinier 6.0 nm Dmax 23.0 nm VolumePorod 436 nm3

SASDCG2 – Rab family protein CtRoco L487A Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16 RgGuinier 5.0 nm Dmax 18.4 nm VolumePorod 447 nm3