Structural characterization of bacterial collagenases

Bauer R
University of Arkansas PhD thesis 2431 (2017 Aug)

URL: https://scholarworks.uark.edu/etd/2431

Submitted to SASBDB: 2017 Apr 8 Published in SASBDB: 2019 Jan 7

SASDCL2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3 Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14 RgGuinier 2.4 nm Dmax 7.5 nm VolumePorod 36 nm3

SASDCM2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 4 Sample: Class1 collagenase collagen-binding domain monomer, 27 kDa Hathewaya histolytica protein Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14 RgGuinier 2.4 nm Dmax 9.8 nm VolumePorod 33 nm3

SASDCN2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 5 Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Sep 14 RgGuinier 2.5 nm Dmax 9.4 nm VolumePorod 36 nm3

SASDCP2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 6 Sample: Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein Buffer: 10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14 RgGuinier 2.6 nm Dmax 12.8 nm VolumePorod 33 nm3