Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.

Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH
Structure 23(5):809-818 (2015 May 5)

PMID: 25817386 doi: 10.1016/j.str.2015.02.012

Submitted to SASBDB: 2017 May 18 Published in SASBDB: 2017 May 5

SASDC73 – Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein Buffer: 20 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 20 RgGuinier 10.3 nm Dmax 34.0 nm VolumePorod 1000 nm3

SASDCL3 – Atg1-Atg13 Subcomplex Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10 RgGuinier 3.3 nm Dmax 10.9 nm VolumePorod 160 nm3

SASDCM3 – Atg17-Atg31-Atg29 Subcomplex Sample: Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10 RgGuinier 10.1 nm Dmax 34.0 nm VolumePorod 848 nm3