Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.

Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M
Angew Chem Int Ed Engl 56(32):9322-9325 (2017 Aug 1)

PMID: 28636238 doi: 10.1002/anie.201702904

Submitted to SASBDB: 2017 May 4 Published in SASBDB: 2017 Jul 24

SASDCC3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA Sample: Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein Poly U 15mer monomer, 5 kDa RNA Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28 RgGuinier 2.4 nm Dmax 8.7 nm VolumePorod 39 nm3

SASDCD3 – Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation) Sample: Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1 RgGuinier 2.7 nm Dmax 10.3 nm VolumePorod 38 nm3

SASDCE3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation) Sample: Poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1 RgGuinier 2.4 nm Dmax 8.6 nm VolumePorod 40 nm3

SASDCF3 – Nucleolysin TIA-1 isoform p40 Sample: Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28 RgGuinier 2.7 nm Dmax 11.2 nm VolumePorod 38 nm3

SASDCG3 – Nucleolysin TIA-1 isoform p40 (LPATG containing construct for sortase mediated protein ligation) Sample: Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4 RgGuinier 2.7 nm Dmax 10.5 nm VolumePorod 37 nm3

SASDCH3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPATG containing construct for sortase mediated protein ligation) Sample: Poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6 Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4 RgGuinier 2.4 nm Dmax 8.6 nm VolumePorod 40 nm3