Ca²⁺ -induced orientation of tandem collagen binding domains from clostridial collagenase ColG permits two opposing functions of collagen fibril formation and retardation.

Caviness P, Bauer R, Tanaka K, Janowska K, Roeser JR, Harter D, Sanders J, Ruth C, Matsushita O, Sakon J
FEBS J 285(17):3254-3269 (2018 Sep)

PMID: 30035850 doi: 10.1111/febs.14611

Submitted to SASBDB: 2017 May 26 Published in SASBDB: 2018 Mar 28

SASDC44 – Envelope of Col H PKD-CBD complexed with mini-collagen Sample: ColH protein monomer, 24 kDa Hathewaya histolytica protein Collagenous Peptide model [(PPG)10] trimer, 9 kDa synthetic construct protein Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 2.7 nm Dmax 12.0 nm VolumePorod 28 nm3

SASDC54 – Envelope of Col H PKD-PKD-CBD complexed with mini-collagen Sample: ColH protein monomer, 34 kDa Hathewaya histolytica protein Collagenous Peptide model [(PPG)10] trimer, 10 kDa synthetic construct protein Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 3.3 nm Dmax 14.2 nm VolumePorod 38 nm3

SASDC64 – Envelope of Col G PKD-CBD-CBD complexed with mini-collagen Sample: Collagenous Peptide model [(PPG)10] trimer, 9 kDa synthetic construct protein ColG Collagenase monomer, 37 kDa Hathewaya histolytica protein Buffer: 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12 RgGuinier 4.1 nm Dmax 19.3 nm VolumePorod 70 nm3