Revealing the distinct folding phases of an RNA three-helix junction.

Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
Nucleic Acids Res 46(14):7354-7365 (2018 Aug 21)

PMID: 29762712 doi: 10.1093/nar/gky363

Submitted to SASBDB: 2017 Jun 2 Published in SASBDB: 2018 May 20

SASDC74 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 0ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.5 nm Dmax 8.0 nm

SASDC94 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.3 nm Dmax 7.2 nm

SASDCA4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 30ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.2 nm Dmax 7.0 nm

SASDCB4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 100ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.3 nm Dmax 7.1 nm

SASDCC4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 300ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.2 nm Dmax 6.8 nm

SASDCD4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 1000ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.2 nm Dmax 6.8 nm

SASDCE4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 3000ms Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3 RgGuinier 2.2 nm Dmax 6.7 nm

SASDCF4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 20mM KCl Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.6 nm Dmax 8.5 nm

SASDCG4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 100mM KCl Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 100mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.5 nm Dmax 8.0 nm

SASDCH4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 200mm KCl Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 200mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.5 nm Dmax 8.0 nm

SASDCJ4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 400mM KCl Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 400mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.4 nm Dmax 8.0 nm

SASDCK4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.25mM MgCl2 Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 20mM KCl 0.25mM MgCl2 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.4 nm Dmax 7.6 nm

SASDCL4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 0.50mM MgCl2 Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 0.5mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.3 nm Dmax 7.2 nm

SASDCM4 – Truncated P5abc subdomain from tetrahymena ribozyme: Static 1mM MgCl2 Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA Buffer: 1mM MgCl2 20mM KCl 10mM KMOPS 20uM EDTA, pH: 7 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 17 RgGuinier 2.2 nm Dmax 7.2 nm