Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.

Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
Nat Commun 8(1):2101 (2017 Dec 13)

PMID: 29235475 doi: 10.1038/s41467-017-02313-6

Submitted to SASBDB: 2017 Jul 10 Published in SASBDB: 2018 Jan 3

SASDC26 – DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 Sample: BCR-ABL p210 fusion protein (DH-PH) monomer, 47 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13 RgGuinier 3.2 nm Dmax 11.1 nm VolumePorod 69 nm3

SASDC36 – DH - Dbl-homology domain of Bcr-Abl tyrosine kinase p210 Sample: BCR-ABL p210 fusion protein monomer, 25 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13 RgGuinier 2.1 nm Dmax 7.3 nm VolumePorod 38 nm3

SASDC46 – PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210 Sample: BCR-ABL p210 fusion protein (PH domain) monomer, 22 kDa Homo sapiens protein Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 10 RgGuinier 2.0 nm Dmax 6.6 nm VolumePorod 38 nm3