Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.

Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G
Structure 25(12):1887-1897.e4 (2017 Dec 5)

PMID: 29107484 doi: 10.1016/j.str.2017.10.001

Submitted to SASBDB: 2017 Sep 1 Published in SASBDB: 2017 Oct 31

SASDCB7 – Streptococcus pneumoniae phosphodiesterase Pde2 Sample: DHH subfamily 1 protein dimer, 70 kDa Streptococcus pneumoniae serotype … protein Buffer: 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23 RgGuinier 2.7 nm Dmax 7.7 nm VolumePorod 87 nm3

SASDCC7 – Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant) Sample: T.maritima PDE dimer, 76 kDa Thermotoga maritima protein Buffer: 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 17 RgGuinier 2.8 nm Dmax 7.9 nm VolumePorod 115 nm3

SASDCD7 – Thermotoga maritima phosphodiesterase TM1595 (wildtype, TmPde) Sample: Thermotoga maritima phosphodiesterase (wildtype, TmPDE, TM1595) dimer, 76 kDa Thermotoga maritima protein Buffer: 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23 RgGuinier 2.7 nm Dmax 7.8 nm VolumePorod 106 nm3