Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion.

Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
Elife 6 (2017 Oct 2)

PMID: 28968219 doi: 10.7554/eLife.29154

Submitted to SASBDB: 2017 Jul 18 Published in SASBDB: 2018 Mar 19

SASDCM6 – Small GTPase Rab5 conjugated with ubiquitin at K116 Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21 RgGuinier 2.6 nm Dmax 8.7 nm

SASDCN6 – Small GTPase Rab5 conjugated with ubiquitin at K140 Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21 RgGuinier 2.3 nm Dmax 8.4 nm

SASDCP6 – Small GTPase Rab5 conjugated with ubiquitin at K165 Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21 RgGuinier 2.7 nm Dmax 9.4 nm