The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome.

Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD
Nucleic Acids Res 46(10):4978-4990 (2018 Jun 1)

PMID: 29850894 doi: 10.1093/nar/gky206

Submitted to SASBDB: 2017 Aug 3 Published in SASBDB: 2018 Mar 25

SASDCT6 – 12N12 nucleosome in 60% sucrose with ADP-BeF3 Sample: 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 4.8 nm Dmax 14.0 nm

SASDCU6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo) Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, pH: 7.8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 5.2 nm Dmax 12.8 nm

SASDCV6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3 Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 5.3 nm Dmax 16.5 nm

SASDCW6 – Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP Sample: Chromodomain-helicase-DNA-binding protein 1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Buffer: 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, 0.5 mM AMP-PNP, pH: 7.8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24 RgGuinier 5.6 nm Dmax 16.7 nm