Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.

Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
Structure 26(2):345-355.e5 (2018 Feb 6)
PMID: 29413323
doi: 10.1016/j.str.2018.01.007 		Submitted to SASBDB: 2017 Aug 9
Published in SASBDB:

SASDCX6 – T2 SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineMechanosensitive channel T2 experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine, 47 kDa synthetic construct
Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
RgGuinier 5.4 nm
Dmax 18.0 nm
VolumePorod 686 nm3

SASDCY6 – T2 in b-DDM micelle (SEC-SAXS)

Mechanosensitive channel T2n-Dodecyl-β-D-Maltopyranoside experimental SAS data
DAMMIF model
Sample: Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
N-Dodecyl-β-D-Maltopyranoside, 77 kDa synthetic construct
Buffer: 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 May 29
RgGuinier 5.6 nm
Dmax 16.5 nm
VolumePorod 896 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine, 47 kDa synthetic construct
Dipeptide and tripeptide permease A monomer, 51 kDa Escherichia coli protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3

SASDC27 – Saposin nanoparticle (SapNP) SapA:DOPC

saposin-a1,2-dioleoyl-sn-glycero-3-phosphocholine experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1,2-dioleoyl-sn-glycero-3-phosphocholine, 47 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
RgGuinier 4.1 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDC37 – Saposin nanoparticle (SapNP) SapA:SoyPI

saposin-aL-α-phosphatidylinositol (soy) experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
L-α-phosphatidylinositol (soy), 52 kDa Glycine max
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
RgGuinier 3.6 nm
Dmax 8.5 nm
VolumePorod 68 nm3

SASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), 46 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
RgGuinier 4.1 nm
Dmax 10.5 nm
VolumePorod 69 nm3