Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.

Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
Structure 26(1):106-117.e6 (2018 Jan 2)

PMID: 29276036 doi: 10.1016/j.str.2017.11.019

Submitted to SASBDB: 2017 Sep 22 Published in SASBDB: 2018 Jan 2

SASDCK7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 13 RgGuinier 2.8 nm Dmax 11.2 nm VolumePorod 61 nm3

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21 RgGuinier 2.8 nm Dmax 9.3 nm VolumePorod 64 nm3

SASDCM7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein Sample: Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein monomer, 61 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 3.3 nm Dmax 11.9 nm VolumePorod 93 nm3

SASDCN7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein Sample: Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein monomer, 62 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 3.4 nm Dmax 13.6 nm VolumePorod 101 nm3

SASDCP7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein Sample: Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein monomer, 72 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 3.8 nm Dmax 14.5 nm VolumePorod 116 nm3

SASDCQ7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein Sample: Grp1 63-399 E161A Arf6 Q67L fusion protein monomer, 60 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15 RgGuinier 3.1 nm Dmax 11.6 nm VolumePorod 86 nm3