Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques.

Liu Z, Gong Z, Cao Y, Ding YH, Dong MQ, Lu YB, Zhang WP, Tang C
Biochemistry 57(3):305-313 (2018 Jan 23)
PMID: 28945353
doi: 10.1021/acs.biochem.7b00817
Submitted to SASBDB: 2017 Sep 12
Published in SASBDB:

SASDCG7 – Lys63-linked diubiquitin at pH7.4

Polyubiquitin-C experimental SAS data
Polyubiquitin-C Kratky plot
Sample: Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Mar 24
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 22 nm3