Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.

Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
Protein Sci 27(3):780-789 (2018 Mar)

PMID: 29266475 doi: 10.1002/pro.3365

Submitted to SASBDB: 2017 Nov 21 Published in SASBDB: 2018 Jan 9

SASDC99 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 100% D2O SANS Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Buffer: PBS in D2O, pH: 7.4 Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25 RgGuinier 3.1 nm Dmax 9.1 nm

SASDCA9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - SAXS Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Buffer: PBS in D2O, pH: 7.4 Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24 RgGuinier 4.8 nm Dmax 12.0 nm

SASDCB9 – Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine, 99 kDa Buffer: PBS in D2O, pH: 7.4 Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2016 Apr 11 RgGuinier 4.3 nm Dmax 10.7 nm

SASDCC9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 42% D2O SANS Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Buffer: PBS in 42% D2O, pH: 7.4 Experiment: SANS data collected at NGB 30m SANS, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Dec 11 RgGuinier 2.9 nm Dmax 7.3 nm

SASDCD9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - SAXS Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Apolipoprotein A-I dimer, 52 kDa Mus musculus protein Buffer: PBS in D2O, pH: 7.4 Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24 RgGuinier 4.7 nm Dmax 12.7 nm

SASDCE9 – Nanolipoprotein Particle (NLP) with Telodendrimer - SAXS Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Apolipoprotein A-I dimer, 52 kDa Mus musculus protein Buffer: PBS in D2O, pH: 7.4 Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24 RgGuinier 4.4 nm Dmax 12.7 nm

SASDCF9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - 100% D2O SANS Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Apolipoprotein A-I dimer, 52 kDa Mus musculus protein Buffer: PBS in D2O, pH: 7.4 Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25 RgGuinier 3.5 nm Dmax 12.5 nm

SASDCG9 – Nanolipoprotein Particle (NLP) with Telodendrimer - 100% D2O SANS Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa Apolipoprotein A-I dimer, 52 kDa Mus musculus protein Buffer: PBS in D2O, pH: 7.4 Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25 RgGuinier 3.3 nm Dmax 10.0 nm