Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex.

Rizzo AA, Vassel FM, Chatterjee N, D'Souza S, Li Y, Hao B, Hemann MT, Walker GC, Korzhnev DM
Proc Natl Acad Sci U S A 115(35):E8191-E8200 (2018 Aug 28)

PMID: 30111544 doi: 10.1073/pnas.1801149115

Submitted to SASBDB: 2017 Nov 14 Published in SASBDB: 2018 Jul 31

SASDCW8 – Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 4mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B monomer, 24 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 5% glycerol, pH: 8.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.1 nm Dmax 6.5 nm VolumePorod 46 nm3

SASDCX8 – Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 6mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B monomer, 24 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 5% glycerol, pH: 8.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.1 nm Dmax 7.6 nm VolumePorod 47 nm3

SASDCY8 – Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 8mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B monomer, 24 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 5% glycerol, pH: 8.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.1 nm Dmax 7.5 nm VolumePorod 46 nm3

SASDCZ8 – Human Rev7 dimer in complex with Rev3 peptide @ 12.2 mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.8 nm

SASDC29 – Human Rev7 dimer in complex with Rev3 peptide @ 10.6 mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.9 nm Dmax 11.0 nm VolumePorod 108 nm3

SASDC39 – Human Rev7 dimer in complex with Rev3 peptide @ 9.1mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 3.0 nm Dmax 11.4 nm VolumePorod 107 nm3

SASDC49 – Human Rev7 dimer in complex with Rev3 peptide @ 6.1mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 3.1 nm Dmax 11.6 nm VolumePorod 105 nm3

SASDC59 – Human Rev7 dimer in complex with Rev3 peptide @ 7.6mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.9 nm Dmax 11.1 nm VolumePorod 106 nm3

SASDC69 – Human Rev7 dimer in complex with Rev3 peptide @ 4.6mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.9 nm

SASDC79 – Human Rev7 dimer in complex with Rev3 peptide @ 3.0mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.8 nm

SASDC89 – Human Rev7 dimer in complex with Rev3 peptide @ 1.5 mg/mL Sample: Mitotic spindle assembly checkpoint protein MAD2B dimer, 49 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein DNA polymerase zeta catalytic subunit monomer, 3 kDa Homo sapiens protein Buffer: 20 mM HEPES, 10 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 14 RgGuinier 2.7 nm