Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.

Trofimova D, Paydar M, Zara A, Talje L, Kwok BH, Allingham JS
Nat Commun 9(1):2628 (2018 Jul 6)
PMID: 29980677
doi: 10.1038/s41467-018-05025-7 		Submitted to SASBDB: 2017 Nov 28
Published in SASBDB:

SASDCR9 – Kif2A-tubulin-DARP complex in the presence of AMP-PNP

Designed Ankyrin Repeat Protein D1Kinesin-like protein KIF2ATubulin alpha-1B chainTubulin beta-2B chain experimental SAS data
DAMMIF model
Sample: Designed Ankyrin Repeat Protein D1 monomer, 18 kDa synthetic construct protein
Kinesin-like protein KIF2A monomer, 48 kDa Homo sapiens protein
Tubulin alpha-1B chain dimer, 100 kDa Bos taurus protein
Tubulin beta-2B chain dimer, 100 kDa Bos taurus protein
Buffer: HEPES 20 mM, MgCl2 1mM, NaCl 150mM, pH: 7.2
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 16
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 374 nm3