Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
PLoS Biol 15(12):e2004486 (2017 Dec)

PMID: 29287065 doi: 10.1371/journal.pbio.2004486

Submitted to SASBDB: 2017 Nov 28 Published in SASBDB: 2018 Jan 3

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19 RgGuinier 2.6 nm Dmax 9.1 nm VolumePorod 60 nm3

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19 RgGuinier 2.9 nm Dmax 9.8 nm VolumePorod 78 nm3