Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.

Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
Proc Natl Acad Sci U S A 114(52):13708-13713 (2017 Dec 26)

PMID: 29229840 doi: 10.1073/pnas.1716614114

Submitted to SASBDB: 2017 Nov 29 Published in SASBDB: 2017 Dec 12

SASDCT9 – Bacteriophage phi-X174 - Empty Capsid Sample: Bacteriophage phi-X174 monomer, 0 kDa protein Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 14.0 nm

SASDCU9 – Bacteriophage phi-X174 - Before Reaction with LPS Sample: Bacteriophage phi-X174 monomer, 0 kDa protein Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 12.1 nm

SASDCV9 – Bacteriophage phi-X174 - ~45s after mixing with LPS Sample: Bacteriophage phi-X174 monomer, 0 kDa protein Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 12.7 nm

SASDCW9 – Bacteriophage phi-X174 - ~245s after mixing with LPS Sample: Bacteriophage phi-X174 monomer, 0 kDa protein Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 12.9 nm

SASDCX9 – Bacteriophage phi-X174 - ~545s after mixing with LPS Sample: Bacteriophage phi-X174 monomer, 0 kDa protein Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4 Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25 RgGuinier 12.9 nm