Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.

Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
J Mol Biol 430(8):1218-1234 (2018 Apr 13)

PMID: 29501381 doi: 10.1016/j.jmb.2018.02.020

Submitted to SASBDB: 2017 Dec 4 Published in SASBDB: 2018 Mar 11

SASDCZ9 – Glyceraldehyde-3-phosphate dehydrogenase from C. reinhardtii Sample: Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 7 RgGuinier 3.2 nm Dmax 9.0 nm VolumePorod 206 nm3

SASDD22 – Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9 Experiment: SAXS data collected at SWING, SOLEIL on 2010 Dec 8 RgGuinier 3.8 nm Dmax 19.0 nm VolumePorod 251 nm3

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein Buffer: 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH: 6.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 3 RgGuinier 2.3 nm Dmax 10.0 nm VolumePorod 22 nm3