Signaling ammonium across membranes through an ammonium sensor histidine kinase.

Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
Nat Commun 9(1):164 (2018 Jan 11)

PMID: 29323112 doi: 10.1038/s41467-017-02637-3

Submitted to SASBDB: 2017 Nov 29 Published in SASBDB: 2018 Jan 16

SASDCM9 – Ammonium transporter Ks-Amt5 Sample: Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 1 RgGuinier 4.8 nm Dmax 17.0 nm VolumePorod 511 nm3

SASDCN9 – Ks-Amt5-HK (apo) Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2 RgGuinier 2.7 nm Dmax 10.0 nm VolumePorod 71 nm3

SASDCP9 – Ks-Amt5-HK (+10uM APPCP) Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2 RgGuinier 2.3 nm Dmax 8.0 nm VolumePorod 53 nm3

SASDCQ9 – Ks-Amt5-HK (+10uM ATP) Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2 RgGuinier 2.2 nm Dmax 7.7 nm VolumePorod 53 nm3

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch) Sample: Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2 RgGuinier 4.9 nm Dmax 17.0 nm VolumePorod 482 nm3