Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems

Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
(2018 Jan 24)

doi: 10.1101/250803

Submitted to SASBDB: 2018 Jan 9 Published in SASBDB: 2018 Jan 24

SASDDQ2 – EspG3 chaperone from Mycobacterium marinum Sample: EspG3 chaperone from Mycobacterium marinum M monomer, 32 kDa Mycobacterium marinum M protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17 RgGuinier 2.3 nm Dmax 8.0 nm

SASDDR2 – EspG1 chaperone from Mycobacterium marinum Sample: EspG1 from Mycobacterium marinum monomer, 30 kDa Mycobacterium marinum protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17 RgGuinier 2.7 nm Dmax 9.7 nm

SASDDS2 – EspG3 chaperone from Mycobacter smegmatis Sample: EspG3 chaperone from Mycobacterium smegmatis monomer, 32 kDa Mycobacterium smegmatis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17 RgGuinier 2.5 nm Dmax 8.6 nm

SASDDT2 – EspG3 chaperone from Mycobacterium tuberculosis Sample: EspG3 chaperone from Mycobacterium tuberculosis, 34 kDa Mycobacterium tuberculosis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17 RgGuinier 2.5 nm Dmax 9.0 nm

SASDDU2 – EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled) Sample: EspG3 chaperone from Mycobacterium smegmatis monomer, 32 kDa Mycobacterium smegmatis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Mar 17 RgGuinier 2.6 nm Dmax 9.2 nm

SASDDV2 – EspG5 chaperone from Mycobacterium tuberculosis Sample: EspG5 chaperone from Mycobacterium tuberculosis monomer, 32 kDa Mycobacterium tuberculosis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jan 12 RgGuinier 2.4 nm Dmax 8.0 nm

SASDDW2 – EspG5-PE25/PPE41 complex from M. tuberculosis Sample: EspG5 chaperone from Mycobacterium tuberculosis monomer, 32 kDa Mycobacterium tuberculosis protein PE25 from Mycobacterium tuberculosis monomer, 11 kDa Mycobacterium tuberculosis protein PPE41 from Mycobacterium tuberculosis monomer, 22 kDa Mycobacterium tuberculosis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Feb 10 RgGuinier 4.0 nm Dmax 13.0 nm

SASDDX2 – EspG3-PE5/PPE4 complex from M. tuberculosis Sample: EspG3 chaperone from Mycobacterium tuberculosis, 34 kDa Mycobacterium tuberculosis protein PE5 from Mycobacterium tuberculosis monomer, 10 kDa Mycobacterium tuberculosis protein PPE4 from Mycobacterium tuberculosis monomer, 52 kDa Mycobacterium tuberculosis protein Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Jun 15 RgGuinier 4.0 nm Dmax 14.2 nm