Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.

Cai K, Frederick RO, Dashti H, Markley JL
Structure 26(8):1127-1136.e4 (2018 Aug 7)

PMID: 29983374 doi: 10.1016/j.str.2018.05.017

Submitted to SASBDB: 2018 Jan 26 Published in SASBDB: 2018 May 16

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex) Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein Acyl carrier protein dimer, 22 kDa Escherichia coli protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22 RgGuinier 3.7 nm Dmax 11.8 nm VolumePorod 204 nm3

SASDDC3 – Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex) Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein Acyl carrier protein dimer, 22 kDa Escherichia coli protein Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22 RgGuinier 3.9 nm Dmax 13.7 nm VolumePorod 218 nm3

SASDDD3 – Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex) Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein Acyl carrier protein dimer, 22 kDa Escherichia coli protein Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein Frataxin, mitochondrial dimer, 29 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 Apr 18 RgGuinier 4.1 nm Dmax 14.4 nm VolumePorod 287 nm3