Conformational analysis of a genetically encoded FRET biosensor by SAXS.

Mertens HD, Piljić A, Schultz C, Svergun DI
Biophys J 102(12):2866-75 (2012 Jun 20)
PMID: 22735537
doi: 10.1016/j.bpj.2012.05.009 		Published in SASBDB:

SASDAE5 – CYNEX4

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) monomer, 93 kDa Homo sapiens protein
Buffer: 50 mM HEPES 150 mM NaCl 1 mM EGTA, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
RgGuinier 3.7 nm
Dmax 12.8 nm
VolumePorod 135 nm3

SASDAF5 – CYNEX4-T266D

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant monomer, 93 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 146 nm3

SASDAJ5 – Annexin-A4

Annexin-A4 experimental SAS data
CRYSOL model
Sample: Annexin-A4 monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Sep 11
RgGuinier 2.4 nm
Dmax 7.6 nm
VolumePorod 54 nm3