Conformational Plasticity of the Immunoglobulin Fc Domain in Solution.

Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M
Structure 26(7):1007-1014.e2 (2018 Jul 3)

PMID: 29731233 doi: 10.1016/j.str.2018.03.017

Submitted to SASBDB: 2018 Mar 6 Published in SASBDB: 2018 Apr 10

SASDDT4 – Fc region of Immunoglobulin G1 (IgG1 Fc) Sample: Immunoglobulin heavy constant gamma 1 dimer, 53 kDa Homo sapiens protein Buffer: 20mM HEPES, 50mM NaCl, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Feb 17 RgGuinier 2.6 nm Dmax 10.0 nm VolumePorod 70 nm3

SASDDU4 – Fc region of Immunoglobulin G2 (IgG2 Fc) Sample: Immunoglobulin heavy constant gamma 2 dimer, 52 kDa Homo sapiens protein Buffer: 20mM HEPES, 50mM NaCl, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Feb 17 RgGuinier 2.8 nm Dmax 9.0 nm VolumePorod 67 nm3

SASDDV4 – Fc-region of Immunoglobulin G1, M135Y/S137T/T139E mutant (IgG1 Fc-YTE) Sample: Immunoglobulin heavy constant gamma 1 M255Y/S257T/T259E dimer, 53 kDa Homo sapiens protein Buffer: 20 mM HEPES, 50mM NaCl, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Feb 17 RgGuinier 2.7 nm Dmax 10.0 nm VolumePorod 74 nm3