Stability of local secondary structure determines selectivity of viral RNA chaperones.

Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R
Nucleic Acids Res (2018 May 18)
PMID: 29796667
doi: 10.1093/nar/gky394 		Submitted to SASBDB: 2018 Apr 1
Published in SASBDB:

SASDDT5 – Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

Nonstructural protein sigma NS20mer RNA (unstructured) experimental SAS data
Nonstructural protein sigma NS 20mer RNA (unstructured) Kratky plot
Sample: Nonstructural protein sigma NS octamer, 325 kDa Avian orthoreovirus protein
20mer RNA (unstructured) dimer, 13 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
RgGuinier 7.8 nm
Dmax 38.0 nm
VolumePorod 964 nm3

SASDDU5 – Nonstructural protein sigma NS - apoprotein

Nonstructural protein sigma NS experimental SAS data
Nonstructural protein sigma NS Kratky plot
Sample: Nonstructural protein sigma NS hexamer, 244 kDa Avian orthoreovirus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
RgGuinier 5.5 nm
Dmax 23.1 nm
VolumePorod 670 nm3