Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α.

Chen KE, Tillu VA, Chandra M, Collins BM
Structure (2018 Sep 19)
PMID: 30293811
doi: 10.1016/j.str.2018.08.010 		Submitted to SASBDB: 2018 Apr 15
Published in SASBDB:

SASDD66 – Phox homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 20
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 43 nm3

SASDD76 – Phox Homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) in complex with inositol-hexaphosphate (IP6)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP 4 mM InsP6, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 20
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 48 nm3